The Relationship of the Properties of Antihemophilic Factor ( Factor VIII ) That Support Ristocetin - Induced Platelet Agglutination
نویسنده
چکیده
A monoclonal antibody to human antihemophilic factor (AHF. factor VIII) was derived from BALB/c mouse spleen cells fused with P3x63Ag8 mouse plasmacytoma cells. This antibody. harvested from culture medium or ascites fluid. reacted with purified AHF and with plasmas from normal subjects or classic hemophiliacs, as measured by enzymelinked immuonsorbant assay (ELISA). but not with plasmas from patients with severe von Willebrand’s disease. The antibody possessed only lgG1 heavy chains and x light chains. It blocked ristocetin-induced platelet agglutination and. to a lesser degree. platelet retention by glass bead columns. but it did not inhibit the procoagulant activity of AHF significantly. An amount of rabbit antiserum against
منابع مشابه
The relationship of the properties of antihemophilic factor (factor VIII) that support ristocetin-induced platelet agglutination (factor VIIIR:RC) and platelet retention by glass beads as demonstrated by a monoclonal antibody.
A monoclonal antibody to human antihemophilic factor (AHF, factor VIII) was derived from BALB/c mouse spleen cells fused with P3x63Ag8 mouse plasmacytoma cells. This antibody, harvested from culture medium or ascites fluid, reacted with purified AHF and with plasmas with normal subjects or classic hemophiliacs, as measured by enzyme-linked immunosorbent assay (ELISA), but not with plasmas from ...
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